Centre for Research University Services (CeSAR)
Biacore X100 SPR System
Biacore X100, Cytiva is a robust and reliable system for the comprehensive label-free analysis and characterization of real time biomolecular interactions. Highly sensitive, real-time detection of molecular binding allows you to carry out new types of studies such as kinetic characterization, highly accurate affinity determination and detection of weak and transient binding events. Biacore X100 Plus Package adds additional layers of functionality to the system, enabling you to perform experiments with molecules in organic solvents, to determine specific binding concentration, and to study biomolecular interactions at physiological temperatures.
Applications
Biacore X100 can be used for a broad range of applications, including structure-function studies, pathway analysis, biomarker discovery and validation, drug target identification and validation, and assay development.
Biacore X100 delivers:
Interaction data with an added dimension: Real-time interaction data enables kinetic characterization, accurate affinity determination and detection of weak and transient binding events.
Versatility: Supports many different assay formats and the study of a wide variety of biomolecules including small molecules, proteins, DNA, and cells. These interactions can be measured in buffer solutions or in crude environments such as serum.
Convenience and efficiency: Workflow-oriented software provides a structured approach to assay development, minimizes hands-on time, and generates reliable data without compromising flexibility for the best assay set up. Preprogrammed workflows for supporting kits, surfaces, and reagents provide solutions for a multitude of assays.
Quality and sensitivity: High quality hardware and software, together with high sensitivity ensure reliable detection of genuine interactions and extend the application range while minimizing sample consumption.
Analysis of kinetic properties: Binding affinity can be resolved into an on-rate, which is primarily driven by molecular recognition, and off-rate, which is controlled by complex stability. This enables a better understanding of molecular interactions and biological processes. Kinetic characterization of interactions can be performed using either the traditional approach, with one sample concentration at a time followed by regeneration of the surface between each analysis cycle, or the more recently developed single-cycle approach. Single-cycle enables you to produce kinetic data in less than an hour from assay development to final data. For capture assays, single-cycle kinetics typically reduces reagent consumption by a factor of four thus reducing cost and time.
Specifiche tecniche
For further information about our SPR services, please contact:
Dr. Rita Pillai
phone: +39 070 675 6587, email: rpillai@unica.it