MoFlo Astrios EQs

The MoFlo AstriosEQs is an High-Speed Cell Sorter system that enable rapid single cell analysis and sorting for both routine testing and further detailed analysis of both single cell and mixed cell populations It features three laser lines (405 nm, 488 nm, and 642 nm), two scatter parameters(side and forward scatter) and 11 fluorescence detectors which can simultaneously separate 6 different cell populations, and enable sterile sorting in excess of 70,000 cells per second. The instrument is housed in an temperature controlled room for sustained stability and has an aerosol containment system.

Technical specifications

Jet-in-air system

Temperature regulation for sample and collection vessels (4°C, 37°C, RT, etc.)

Available nozzle sizes: 70um, 100um

Up to 6 populations can be sorted at the same time (only available for 5mL tubes)

Various collection vessels can be used:

5mL, 2mL, 1.5mL tubes (6-way sort possible)
15mL tubes (2-way sort)
50mL tubes (2-way sort)
slides
384w, 96w, 48w, 24w,12w, 6w plates (1-way sort)
PCR tubes/strips/plates (1-way sort)

Sort setup modes: Purity, Single Cell (cloning), Enrichment

Configuration

Violet Laser 405 nm	Fluorocromes	Blue Laser 488 nm	Fluorocromes	Red Laser 642 nm	Fluorocromes
448/59	VK 405, DAPI, PB	513/26	FITC; AF488	671/30	APC; DRAQ7
546/20	KO; PO	576/20	PE; RPE	722/44	APC-Cy5.5; AF700
		620/29	PE-Texas Red; PI	795/70	APC-Cy7: APC-AF750
		644/22	7AAD; PercP
		710/45	PC5.5; PE-AF700
		795/70	PC7
		488/6
		SSC

Applications

The facility offers different level of services according to the user’s preferences:

Consultation regarding the experimental design;

Design and implementation of comprehensive studies;

Apoptosis/necrosis analysis;

Cell cycle;

PBMC and red blood immunophenotyping;

Cell sorting to obtain purified cell populations based on the immunophenotype and/or functional features, including:

Purification of rare cells from a heterogeneous sample.
Sorting eukaryotic and procaryotic cells.
High-purity cell sorting up to 6 cell populations
Optimized recovery of intact/viable cells for further analysis such as PCR, DNA sequencing, cell culture, use in animal models, etc.

For further information about our Flow Cytometry and Cell sorting services, please contact: cesar@unica.it

Dr. Rita Pillai

phone: +39 070 675 6587, email: rpillai@unica.it

Centre for Research University Services (CeSAR)

Centre for Research University Services (CeSAR)