Droplet digital PCR
The lab is equipped with the Bio-Rad QX200 droplet digital PCR system, which provides extremely accurate quantification of DNA. It uses limiting dilutions of the target DNA in up to 20,000 sub-nanoliter droplets. The instrument can use:
· Taqman assays with two color detection (FAM and VIC/HEX) on a single sample.
· Evagreen assays with only a pair of PCR primers.
It is used for applications such as:
· Rare mutation detection
· viral load detection
· gene expression studies
· single-cells genotyping
· Environmental monitoring
· Food testing
· CNV analyses
· NGS library quantification