Droplet digital PCR

Bio-Rad QX200

The lab is equipped with the Bio-Rad QX200 droplet digital PCR system, which provides extremely accurate quantification of DNA. It uses limiting dilutions of the target DNA in up to 20,000 sub-nanoliter droplets. The instrument can use:

·        Taqman assays with two color detection (FAM and VIC/HEX) on a single sample.

·        Evagreen assays with only a pair of PCR primers.

It is used for applications such as:

·        Rare mutation detection

·        viral load detection

·        gene expression studies

·        single-cells genotyping

·        Environmental monitoring

·        Food testing

·        CNV analyses

·        NGS library quantification

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