Immuno-electrophysiology on neuromuscular junctions of drosophila third instar larva

Klima R.;Romano G.;Gbadamosi M.;Megighian A.;Feiguin F.

Ultimo

2021-01-01

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Abstract

Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through immune-electrophysiology in Drosophila melanogaster. This technique allows the quantification of the molecular behavior of the neuromuscular synapses by correlating the structural configuration of the synaptic boutons with their electrical activity.