Immuno-electrophysiology on neuromuscular junctions of drosophila third instar larva

Feiguin F.
Last
2021-01-01

Abstract

Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through immune-electrophysiology in Drosophila melanogaster. This technique allows the quantification of the molecular behavior of the neuromuscular synapses by correlating the structural configuration of the synaptic boutons with their electrical activity.
2021
Inglese
BIO-PROTOCOL
11
3
e3913
17
WOS:000616641600013
2-s2.0-85116232936
33732800
Esperti anonimi
internazionale
scientifica
Drosophila melanogaster; Electrophysiology; Immunocytochemistry; Neurological disorders; Neuromuscular synapses; Synaptic boutons; Third instar larvae
no
Klima, R.; Romano, G.; Gbadamosi, M.; Megighian, A.; Feiguin, F.
1.1 Articolo in rivista
info:eu-repo/semantics/article
1 Contributo su Rivista::1.1 Articolo in rivista
262
5
reserved
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