Usefulness of capillary electrophoresis-based multiplex PCR assay for species-specific identification of Candida spp.

Mallus F;Martis S;SERRA, CORRADO;Loi G;Camboni T;MANZIN, ALDO

2013-01-01

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Abstract

The study evaluated the performances of a commercial multiplex PCR assay, the Seegene Seeplex STI Master Panel 3, for Candida spp. identification. Eighty clinical strains of Candida spp. were identified with this system and a homemade multiplex PCR assay. The results were also compared with those obtained with two phenotypic methods. The study provided a preliminary evaluation of a multiplex assay from Seegene that uses capillary electrophoresis as the detection of amplified products. The Seeplex assay was found to be a rapid and useful method for identifying large numbers of yeast isolates in the clinical laboratory context.