Isolation of monomeric photosystem II that retains the subunit PsbS

Haniewicz P.;De Sanctis D.;Büchel C.;Schröder W. P.;LOI, MARIA CECILIA;Kieselbach T.;Bochtler M.;PIANO, DARIO

2013-01-01

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Abstract

Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221-226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A "milder" procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501-1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants.