In vitro imaging of β-cells using fluorescent cubic bicontinuous liquid crystalline nanoparticles

Miceli, V.;MELI, VALERIA;Blanchard Desce, M.;Bsaibess, T.;Pampalone, M.;Conaldi, P. G.;CALTAGIRONE, CLAUDIA;Obiols Rabasa, M.;Schmidt, J.;Talmon, Y.;Casu, A;MURGIA, SERGIO

2016-01-01

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Abstract

To evaluate the potential of monoolein-based cubic bicontinuous liquid crystalline nanoparticles (cubosomes) as diagnostic tools in diabetes and pancreatic β-cell transplantation, fluorescent cubosomes were formulated, entrapping within the lipid bilayer a newly synthesized, potent, hydrophobic dye. Cryo-TEM, SAXS, and DLS were performed to assess the morphological and structural aspects of this formulation. Rat pancreatic β-cells (INS-1E) readily took up the cubosome nanoparticles, as revealed by in vitro internalization tests, and flow-cytometric analyses confirmed the persistence of the fluorescence for up to 24 hours. The cytotoxicity of cubosomes at various concentrations, evaluated with a real-time analysis of proliferation, revealed that the proliferation curves relative to the less concentrated formulation (5 μM in terms of monoolein content) were comparable to the untreated cultures. INS-1E cell cycle and apoptosis supported such results. In conclusion, this formulation, with dye content in the nanomolar range, seems to be useful for β-cell imaging.