The Salivary Proteome Profile in Patients Affected by SAPHO Syndrome characterized by a top-down RP-HPLC-ESI-MS platform

SANNA, MONICA;FIRINU, DAVIDE;MANCONI, PAOLO EMILIO;DEL GIACCO, STEFANO;Pisanu M;PIRAS, VALENTINA;Castagnola M;Messana I;Cabras T.

2014-01-01

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Abstract

SAPHO syndrome is a rare and often unrecognized disease with prominent inflammatory cutaneous and articular manifestations characterized by similar musculoskeletal manifestations (synovitis, hyperostosis, osteomyelitis) associated with dermatological conditions (severe acne and pustulosis) [1]. The acidic soluble fraction of whole saliva from 10 adult women affected by SAPHO syndrome and from 10 sex- and age-matched healthy subjects was analysed by RP-HPLC-ESI-MS with the aim to discover salivary biomarkers of the disorder. The levels of oral proteins and peptides were correlated with clinical data. The following proteins showed a significant decreased concentration in saliva of SAPHO subjects with respect to controls: cystatin S1 and S2, histatin 1 and 5 and the major acidic PRPs. Salivary concentration of cystatin S1 and S2 positively correlated with neutrophil count in patients, and the abundance of cystatin S2 lowered according to the disease duration. Histatin 1 and 5 showed positive relationship with C reactive protein and negative with the disease duration. Levels and frequency of S100A12 protein showed a trend to increase in SAPHO patients. The high expression of this pro-inflammatory protein is probably related to the inflammatory response and to the altered neutrophil responses to functional stimuli that characterize SAPHO syndrome [2] and it is in agreement with previous immunohistochemical studies, which demonstrated the strong production of S100A12 in synovial tissue of patients affected by psoriatic and rheumatoid arthritis [3].