The impact of StuI digestion in situ on FISH to human chromosomes with satellite DNA probes

NIEDDU, MARIELLA;PICHIRI, GIUSEPPINA;MELIS V;MEZZANOTTE R.

2003-01-01

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Abstract

Human metaphase chromosomes were digested with Stul and subsequently hybridized in situ using chromosome 9 alphoid DNA and classical satellite III DNA as probes. The data obtained suggest that it is not possible to establish a general rule regarding the cytological effects induced by restriction enzymes in particular chromosome regions and that a number of factors, such as DNA sequences, DNA-protein interaction and enzyme structure, play a role in determining such effects.