Heligmosomoides polygyrus bakeri infection decreases Smad7 expression in intestinal CD4+ T cells, which allows TGF-b to induce IL-10–producing regulatory T cells that block colitis

Hang L.;Kumar S.;Blum A. M.;Urban J. F.;Fantini M. C.;Weinstock J. V.

2019-01-01

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Abstract

Helminthic infections modulate host immunity and may protect their hosts from developing immunological diseases like inflammatory bowel disease. Induction of regulatory T cells (Tregs) may be an important part of this protective process. Heligmosomoides polygyrus bakeri infection also promotes the production of the regulatory cytokines TGF-beta and IL-10 in the gut. In the intestines, TGF-beta helps induce regulatory T cells. This study used Foxp3/IL-10 double reporter mice to investigate the effect of TGF-beta on the differentiation of colon and mesenteric lymph node-derived murine Foxp3(-) IL-10(-) CD4(+) T cells into their regulatory phenotypes. Foxp3(-) IL-10(-) CD4(+) T cells from H. polygyrus bakeri-infected mice, as opposed to T cells from uninfected animals, cultured in vitro with TGF-beta and anti-CD3/CD28 mAb differentiated into Foxp3(+) and/or IL-10(+) T cells. The IL-10-producing T cells nearly all displayed CD25. Smad7 is a natural inhibitor of TGF-beta signaling. In contrast to gut T cells from uninfected mice, Foxp3(-) IL10(-) CD4(+) T cells from H. polygyrus bakeri- infected mice displayed reduced Smad7 expression and responded to TGF-beta with Smad2/3 phosphorylation. The TGF-beta-induced Tregs that express IL-10 blocked colitis when transferred into the Rag/CD25(-) CD4(+) T cell transfer model of inflammatory bowel disease. TGF-beta had a greatly diminished capacity to induce Tregs in H. polygyrus bakeri-infected transgenic mice with constitutively high T cell-specific Smad7 expression. Thus, infection with H. polygyrus bakeri causes down-modulation in Smad7 expression in intestinal CD4(+) T cells, which allows the TGF-beta produced in response to the infection to induce the Tregs that prevent colitis.