Degradation of juglone by Pleurotus sajor-caju

CURRELI, NICOLETTA;RESCIGNO, ANTONIO;Rinaldi A;Pisu B;Sollai F;SANJUST, ENRICO

2004-01-01

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Abstract

The toxic naphthoquinone juglone (5-liydroxy-1,4-naphthoquitione) is efficiently degraded by the ligninolytic fungus Pleurotus sajor-caju, as demonstrated by the total bleaching within 9 d of a conventional liquid culture medium Supplemented with 0.6 mM juglone. The oxidative degradation involves the production of hydrogen peroxide arising from both enzymic and non-enzymic oxidation reactions, promoted by the fungus. Juglone is not directly attacked bv the oxidative enzymes of the ligninolytic machinery of P. saior-caju, such as laccase, manganese peroxidase and arvIalcohol oxidase. On the other hand, this naphthoquinone is a good substrate for a reductase, which triggers ail auto-oxidative process producing reactive oxygen species and leading to juglone degradation. The degradation process continues to completion by means of a direct, presumably non-catalysed reaction with hydrogen peroxide.