Diafiltration in the presence of ascorbate in the purification of mushroom tyrosinase

RESCIGNO, ANTONIO;SANJUST, ENRICO;RINALDI, ANDREA;CURRELI, NICOLETTA;
1997-01-01

Abstract

In the present paper a method for the extraction of Agaricus bisporus tyrosinase, based on the use of a diafiltration procedure in the presence of ascorbate, is proposed. During the extraction of tyrosinase the oxidation of mushroom phenolics was avoided, keeping phenolic compounds in their reduced form throughout the extraction step. Their complete removal by means of a diafiltration apparatus was achieved. The method here described represents a useful way to obtain an enzymic solution of tyrosinase, devoid of phenolics and melanic compounds.
1997
Inglese
PHYTOCHEMISTRY
46
1
21
22
2
WOS:A1997XR57300004
2-s2.0-0030752124
http://www.sciencedirect.com/science/article/pii/S003194229600739X
http://apps.webofknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=9&SID=W1hgbDpnNCmLkGBo@aE&page=1&doc=41
Esperti anonimi
Tyrosinase; Protein purification; Agaricus bisporus
Rescigno, Antonio; F., Sollai; Sanjust, Enrico; Rinaldi, Andrea; Curreli, Nicoletta; A., Rinaldi
1.1 Articolo in rivista
info:eu-repo/semantics/article
1 Contributo su Rivista::1.1 Articolo in rivista
262
6
none
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