Top-down proteomic profiling of human saliva in multiple sclerosis patients
Barbara Manconi
;Tiziana Cabras;Lorena Lorefice;Eleonora Cocco;Alessandra Olianas
2018-01-01
Abstract
Multiple sclerosis is a chronic disease of the central nervous system characterized by inflammation, demyelination and neurodegeneration which is of undetermined origin. To date a single diagnostic test of multiple sclerosis does not exists and novel biomarkers are demanded for a more accurate and early diagnosis. In this study, we performed the quantitative analysis of 119 salivary peptides/proteins from 49 multiple sclerosis patients and 54 healthy controls by a mass spectrometry-based top-down proteomic approach. Statistical analysis evidenced different levels on 23 proteins: 8 proteins showed lower levels in multiple sclerosis patients with respect to controls and they were mono- and di-oxidized cystatin SN, mono- and di-oxidized cystatin S1, mono-oxidized cystatin SA and mono-phosphorylated statherin. 15 proteins showed higher levels in multiple sclerosis patients with respect to controls and they were antileukoproteinase, two proteoforms of Prolactin-Inducible Protein, P-C peptide (Fr.1-14, Fr. 26-44, and Fr. 36-44), SV1 fragment of statherin, cystatin SN Des1-4, cystatin SN P11 → L variant, and cystatin A T96 → M variant. The differences observed between the salivary proteomic profile of patients suffering from multiple sclerosis and healthy subjects is consistent with the inflammatory condition and altered immune response typical of the pathology. Data are available via ProteomeXchange with identifier PXD009440.File | Size | Format | |
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Multiple slerosis.pdf Solo gestori archivio
Description: main document
Type: versione editoriale
Size 1.85 MB
Format Adobe PDF
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1.85 MB | Adobe PDF | & nbsp; View / Open Request a copy |
licensed_pagination_JPROT_3188.pdf open access
Type: Author’s Accepted Manuscript AAM, Post-print, (version accepted by the publisher)
Size 1.08 MB
Format Adobe PDF
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1.08 MB | Adobe PDF | View/Open |
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