Aula A, Dipartimento di Fisica, 14 Dicembre 2010, 15:00

 

"Cryo-EM investigations of ribosome assembly"

 

Institut für Organische Chemie / AK Fucini Germany

 

In Escherichia coli approximately 40% of the cellular energy is devoted to protein synthesis, a process that is carried out by the ribosome.

Accordingly, the synthesis and activity of the ribosome is a highly coordinated process such that this significant energy expenditure is

balanced with environmental conditions. RsgA (ribosome small subunit-

dependent GTPase A) is an extra-ribosomal protein that is presumed to play a role in ribosome assembly and/or regulating the process of protein synthesis. We will present a preliminary cryo-EM analysis of a complex with RsgA bound to the E. coli 30S subunit.