Improvement of quercetin protective effect against oxidative stress skin damages by incorporation in nanovesicles

MANCA, MARIA LETIZIA;Castangia, I;CADDEO, CARLA;VALENTI, DONATELLA;LAMPIS, SANDRINA;FADDA, ANNA MARIA;MANCONI, MARIA
2014-01-01

Abstract

Quercetin was incorporated in glycerosomes, new phospholipid-glycerol vesicles, and their protective effect against oxidative stress skin damages was extensively evaluated. In particular, the concentration-dependent effect of glycerol (from 10 to 50%) on vesicle suitability as cutaneous carriers of quercetin was carefully assessed. All vesicles were unilamellar and small in size (∼80-110. nm), as confirmed by cryo-TEM observation, with a drug incorporation efficiency ranging between 81 and 91%. SAXS studies, performed to investigate the bilayer arrangement, indicated a strong, dose-dependent interaction of glycerol with the polar portions of the phospholipid molecules, while quercetin did not significantly change the bilayer packing. In vitro studies on newborn pig skin underlined the concentration-dependent ability of glycerosomes to promote quercetin accumulation in the different layers, also confirmed by confocal microscopic observation of skin treated with fluorescent vesicles. Quercetin incorporated into liposomal and glycerosomal nanoformulations showed a strong ability to scavenge free radicals (DPPH test) and protect human keratinocytes in vitro against hydrogen peroxide damage. Moreover, quercetin-loaded vesicles were avidly taken up by keratinocytes in vitro.Overall, results indicate 40 and 50% glycerosomes as promising nanosystems for the improvement of cutaneous quercetin delivery and keratinocyte protection against oxidative stress damage.
2014
Inglese
123
566
574
9
Esperti anonimi
internazionale
scientifica
Manca, MARIA LETIZIA; Castangia, I; Caddeo, Carla; Pando, D; Escribano, E; Valenti, Donatella; Lampis, Sandrina; Zaru, M; Fadda, ANNA MARIA; Manconi, ...espandi
1.1 Articolo in rivista
info:eu-repo/semantics/article
1 Contributo su Rivista::1.1 Articolo in rivista
262
10
reserved
Files in This Item:
File Size Format  
Quercetina glicerosomi 2014.pdf

Solo gestori archivio

Type: versione editoriale
Size 3.15 MB
Format Adobe PDF
3.15 MB Adobe PDF & nbsp; View / Open   Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Questionnaire and social

Share on:
Impostazioni cookie