Lysozyme Adsorption and Release from Ordered Mesoporous Materials

Bhattacharyya MS;Hiwale P;Piras M;MEDDA, LUCA;STERI, DANIELA;PILUDU, MARCO;SALIS, ANDREA;MONDUZZI, MAURA

2010-01-01

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Abstract

Ordered mesoporous materials (OMMs) are interesting matrixes for nanomedicine applications such as innovative drug delivery systems. Here, we compare the behavior of the widely studied SBA-15 mesoporous silica with that of the less investigated MSE (a periodic mesoporous organosilica whose silicon atoms are alternatively connected by means of -Si-O-Si- and -Si-CH(2)-CH(2)-Si- groups) toward the adsorption (pH 7.0 and 9.6) and in vitro release (pH 7.4; T = 37 degrees C) of an antimicrobial protein (hen egg white lysozyme). Both OMMs have a hexagonal ordered mesoporous structure and texture, as confirmed by SAXS, TEM, and N(2) adsorption isotherms, but differ for the chemical composition and surface charge density, as determined by ATR-FTIR spectroscopy and potentiometric titrations, respectively. Rather than the structural and textural features, the different chemical composition of SBA-15 and MSE seems to be responsible for the different lysozyme loading and release and for the different stability toward the lixiviating action of the physiological medium (pH 7.4; T = 37 degrees C).