Computational Design of a DNA- and Fc-Binding Fusion Protein

ARMANO, GIULIANO;
2011-01-01

Abstract

Computational design of novel proteins with well-defined functions is an ongoing topic in computational biology. In this work, we generated and optimized a new synthetic fusion protein using an evolutionary approach. The optimization was guided by directed evolution based on hydrophobicity scores, molecular weight, and secondary structure predictions. Several methods were used to refine the models built from the resulting sequences. We have successfully combined two unrelated naturally occurring binding sites, the immunoglobin Fc-binding site of the Z domain and the DNA-binding motif of MyoD bHLH, into a novel stable protein.
2011
Inglese
2011
457578
7
http://www.hindawi.com/journals/abi/2011/457578/
Esperti anonimi
Winkler, J; Armano, Giuliano; Dybowski J., N; Kuhn, O; Ledda, F; Heider, D.
1.1 Articolo in rivista
info:eu-repo/semantics/article
1 Contributo su Rivista::1.1 Articolo in rivista
262
6
none
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