Antibodies targeting the European lobster (Palinurus elephas) vitellogenin developed by mRNA isolation and in-silico-designed antigenic peptides

Cannea, Faustina B

First

Conceptualization

;Follesa, Cristina

Second

Conceptualization

;Porcu, Cristina

Conceptualization

;Rossino, Rossano

Resources

;Olianas, Alessandra

Validation

;Rescigno, Antonio

Penultimate

Writing - Review & Editing

;Padiglia, Alessandra

Last

Writing - Original Draft Preparation

2022-01-01

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Abstract

Vitellogenin is an essential protein involved in ovary maturation in many animals. Detection of this protein correlated with reproductive capacity may be important if carried out on marine organisms such as the red spiny lobster Palinurus elephas, a crustacean economically important crop from wild fish catches. Moreover, in recent years, vitellogenin has assumed an important role as a possible biomarker of marine environmental pollution, as its expression levels can be influenced by the presence of similar oestrogen pollutants and affect the reproductive sphere of marine organisms such as crustaceans. The P. elephas vitellogenin protein and its coding gene have never been isolated, so there is little information about its presence in this lobster. The aim of the present study was to develop a molecular strategy to create, for the first time, an antibody for the detection and quantization of vitellogenin in P. elephas.