A population genomics insight by 2b‐RAD reveals populations' uniqueness along the Italian coastline in Leptopsammia pruvoti (Scleractinia, Dendrophylliidae)

Boscari, Elisa;Abbiati, Marco;Badalamenti, Fabio;Bavestrello, Giorgio;Benedetti‐Cecchi, Lisandro;Cannas, Rita;Cau, Angelo;Cerrano, Carlo;Chimienti, Giovanni;Costantini, Federica;Fraschetti, Simonetta;Ingrosso, Gianmarco;A. M. Marino, Ilaria;Mastrototaro, Francesco;Papetti, Chiara;Paterno, Marta;Ponti, Massimo;Zane, Lorenzo;Congiu, Leonardo

2019-01-01

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Abstract

Aim: Marine bioconstructions such as coralligenous formations are hotspot of biodi‐ versity and play a relevant ecological role in the preservation of biodiversity by provid‐ ing carbon regulation, protection and nursery areas for several marine species. For this reason, the European Union Habitat Directive included them among priority habitats to be preserved. Although their ecological role is well established, connectivity pat‐ terns are still poorly investigated, representing a limit in conservation planning. The present study pioneers a novel approach for the analysis of connectivity in marine bioconstructor species, which often lack suitable genetic markers, by taking advantage of next‐generation sequencing techniques. We assess the geographical patterns of genomic variation of the sunset cup coral Leptopsammia pruvoti Lacaze‐Duthiers, 1897, an ahermatypic, non‐zooxanthellate and solitary scleractinian coral species common in coralligenous habitats and distributed across the Mediterranean Sea. Location: The Italian coastline (Western and Central Mediterranean). Methods: We applied the restriction site‐associated 2b‐RAD approach to genotype over 1,000 high‐quality and filtered single nucleotide polymorphisms in 10 population samples. Results: The results revealed the existence of a strongly supported genetic structure, with highly significant pairwise FST values between all the population samples, includ‐ ing those collected about 5 km apart from each other. Moreover, genomic data indi‐ cate that the strongest barriers to gene flow are between the western (Ligurian–Tyrrhenian Sea) and the eastern side (Adriatic Sea) of the Italian peninsula. Main conclusions: The strong differentiation found in L. pruvoti is similar to that found in other species of marine bioconstructors investigated in this area, but it strongly contrasts with the small differences found in many fish and invertebrates at the same geographical scale. All in one, our results highlight the importance of assessing con‐ nectivity in species belonging to coralligenous habitats as, due to their limited disper‐ sal ability, they might require specific spatial conservation measures.