Getting drugs through small pores: Exploiting the porins pathway in Pseudomonas aeruginosa

Susruta Samanta;Igor Bodrenko

Co-prime

;Silvia Acosta-Gutieŕrez;Tommaso D’Agostino;Monisha Pathania;Ishan Ghai;Christian Schleberger;Dirk Bumann;Richard Wagner;Mathias Winterhalter;Bert van den Berg;Matteo Ceccarelli

Last

2018-01-01

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Abstract

Understanding molecular properties of outer membrane channels of Gram-negative bacteria is of fundamental significance as they are the entry point of polar antibiotics into bacteria. Outer membrane proteomics revealed OccK8 (OprE) to be among the five most expressed substrate specific channels of the clinically important Pseudomonas aeruginosa. The high-resolution X-ray structure and electrophysiology highlighted a very narrow pore. However, experimental in vitro methods showed the transport of natural amino acids and antibiotics, among them ceftazidime. We used molecular dynamics simulations to reveal the importance of the physico-chemical properties of ceftazidime in modulating the translocation through OccK8, proposing a structure-function relationship. As in general porins, the internal electric field favors the translocation of polar molecules by gainful energy compensation in the central constriction region. Importantly, the comparatively narrow Occk8 pore can undergo a substrate-induced expansion to accommodate relatively large-sized substrates.