Effect of site-directed mutations in multidrug efflux pump AcrB examined by quantitative efflux assays

Kinana, Alfred D;VARGIU, ATTILIO VITTORIO;Nikaido, Hiroshi

2016-01-01

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Abstract

The Resistance-Nodulation-Division (RND) family transporter AcrB plays a major role in the intrinsic and increased resistance of Escherichia coli to a large number of antibiotics. The distal binding pocket within this multidrug efflux transporter is very large, but the effort to define the roles of various residues facing this pocket through site-directed mutagenesis so far involved only the determination of minimal inhibitory concentrations of drugs in mutants.