Eleonora Fois Fois
Docetaxel-loaded fluorescent liquid-crystalline nanoparticles for cancer theranostics
MELI, VALERIA;CALTAGIRONE, CLAUDIA;FALCHI, ANGELA MARIA;LIPPOLIS, VITO;MONDUZZI, MAURA;ROSA, ANTONELLA;MURGIA, SERGIO
2015-01-01
Abstract
Here, we describe a novel monoolein-based cubosome formulation engineered for possible theranostic applications in oncology. The Docetaxel-loaded nanoparticles were stabilized in water by a mixture of commercial Pluronic (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer) F108 (PF108) and rhodamine- and folate-conjugated PF108 so that the nanoparticles possess targeting, therapeutic, and imaging properties. Nanoparticles were investigated by DLS, cryo-TEM, and SAXS to confirm their structural features. The fluorescent emission characterization of the proposed formulation indicated that the rhodamine conjugated to the PF108 experiences an environment less polar than water (similar to chloroform), suggesting that the fluorescent fragment is buried within the poly(ethylene oxide) corona surrounding the nanoparticle. Furthermore, these nanoparticles were successfully used to image living HeLa cells and demonstrated a significant short-term (4 h incubation) cytotoxicity effect against these cancer cells. Furthermore, given their analogy as nanocarriers for molecules of pharmaceutical interest and to better stress the singularities of these bicontinuous cubic nanoparticles, we also quantitatively evaluated the differences between cubosomes and multilamellar liposomes in terms of surface area and hydrophobic volume.File | Size | Format | |
---|---|---|---|
Murgia_Langmuir 2015.pdf Solo gestori archivio
Type: versione editoriale
Size 3.74 MB
Format Adobe PDF
|
3.74 MB | Adobe PDF | & nbsp; View / Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.