Purification and characterization of DR_2577 (SlpA) a major S-layer protein from Deinococcus radiodurans

Farci, D;Bowler, MW;ESPOSITO, FRANCESCA;McSweeney, S;TRAMONTANO, ENZO;PIANO, DARIO

2015-01-01

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Abstract

The protein DR_2577 is a major Surface layer component of the radio-resistant bacterium Deinococcus radiodurans. In the present study DR_2577 has been purified and its oligomeric profile characterized by means of size exclusion chromatography and gel electrophoresis. DR_2577 was found to be organized into three hierarchical orders characterized by monomers, stable dimers formed by the occurrence of disulfide bonds, and hexamers resulting from a combination of dimers. The structural implications of these findings are discussed providing new elements for a more integrated model of this S-layer.