Cubosome formulations stabilized by a dansyl-conjugated block copolymer for possible nanomedicine applications

MURGIA, SERGIO;FALCHI, ANGELA MARIA;Meli V;Schillen K;LIPPOLIS, VITO;MONDUZZI, MAURA;ROSA, ANTONELLA;Schmidt J;Talmon Y;Bizzarri R;CALTAGIRONE, CLAUDIA

2015-01-01

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Abstract

We present here an innovative, fluorescent, monoolein-based cubosome dispersion. Rather than embedded within the monoolein palisade, the fluorescent imaging agent, namely dansyl, was conjugated to the terminal ethylene oxide moieties of the block copolymer Pluronic F108. We discuss the physicochemical and photophysical properties of this fluorescent Pluronic and of a cubosome formulation stabilized by a mixture of dansyl-conjugated and non-conjugated Pluronic, also including an anticancer drug (quercetin). Furthermore, we performed biocompatibility tests against HeLa cells to assess internalization and cytotoxicity features of this nanoparticles aqueous dispersion. Cryo-TEM, SAXS, and DLS analysis, proved the bicontinuous cubic inner nanostructure and the morphology of this fluorescent cubosome dispersion, while photophysical measurements and biocompatibility results basically validate their potential use for theranostic nanomedicine applications.