New generation QuIC assays for prion seeding activity
ORRU', CHRISTINA DORIANACo-prime
;VASCELLARI, SARAH;
2012-01-01
Abstract
The ability of abnormal TSE-associated forms of PrP to seed the formation of amyloid fibrils from recombinant PrPSen has served as the basis for several relatively rapid and highly sensitive tests for prion diseases. These tests include rPrP-PMCA (rPMCA), standard quaking-induced conversion (S-QuIC), amyloid seeding assay (ASA), real-time QuIC (RT-QuIC) and enhanced QuIC (eQuIC). Here, we summarize recent improvements in the RT-QuIC-based assays that enhance the practicality, sensitivity and quantitative attributes of assays QuIC and promote the detection of prion seeding activity in dilute, inhibitor-laden fluids such as blood plasma.File | Size | Format | |
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Prion 2012 Landes Bioscience.pdf Solo gestori archivio
Type: versione editoriale
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