Excitation polarization provides structural resolution of individual non-blinking nano-objects

Thomsson D

First

;Sforazzini G

Second

;Anderson HL;Scheblykin IG

2013-01-01

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Abstract

We propose to combine the method of fluorescence intensity centroid localization with rotation of the plane of excitation polarization. Polarized light interacts selectively with differently oriented fluorophores; thus yielding topological information on the nanometer scale, without any need for fluorophore blinking. The method is applicable to photostable individual systems, when most of the traditional super-resolution methods fail. A theoretical study is supported by experiments on 30 nm long cyclodextrin-encapsulated single polyrotaxane conjugated polymer chains.