Metabolomic alterations in thyrospheres and adherent parental cells in papillary thyroid carcinoma cell lines: A pilot study
Caria PFirst
Writing - Original Draft Preparation
;Tronci LSecond
Writing - Original Draft Preparation
;Dettori TMember of the Collaboration Group
;Murgia FMember of the Collaboration Group
;Santoru MLMember of the Collaboration Group
;Griffin JLWriting - Original Draft Preparation
;Vanni R
Penultimate
Writing - Original Draft Preparation
;Atzori LLast
Writing - Original Draft Preparation
2018-01-01
Abstract
Papillary thyroid carcinoma (PTC), is characterized by a heterogeneous group of cells, including cancer stem cells (CSCs), crucially involved in tumor initiation, progression and recurrence. CSCs appear to have a distinct metabolic phenotype, compared to non-stem cancer cells. How they adapt their metabolism to the cancer process is still unclear, and no data are yet available for PTC. We recently isolated thyrospheres, containing cancer stem-like cells, from B-CPAP and TPC-1 cell lines derived from PTC of the BRAF-like expression profile class, and stem-like cells from Nthy-ori3-1 normal thyreocyte-derived cell line. In the present study, gas chromatography/mass spectrometry metabolomic profiles of cancer thyrospheres were compared to cancer parental adherent cells and to non cancer thyrospheres profiles. A statistically significant decrease of glycolytic pathway metabolites and variations in Krebs cycle metabolites was found in thyrospheres versus parental cells. Moreover, cancer stem-like cells showed statistically significant differences in Krebs cycle intermediates, amino acids, cholesterol, and fatty acids content, compared to non-cancer stem-like cells. For the first time, data are reported on the metabolic profile of PTC cancer stem-like cells and confirm that changes in metabolic pathways can be explored as new biomarkers and targets for therapy in this tumor.File | Size | Format | |
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Metabolomic Alterations in Thyrospheres andijms-19-02948-v2.pdf open access
Type: versione editoriale
Size 2.46 MB
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2.46 MB | Adobe PDF | View/Open |
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